Signal Recognition ParticleAluDomain Occupies a Defined Site at the Ribosomal Subunit Interface upon Signal Sequence Recognition†
نویسندگان
چکیده
منابع مشابه
Signal recognition particle Alu domain occupies a defined site at the ribosomal subunit interface upon signal sequence recognition.
The eukaryotic signal recognition particle (SRP) is essential for cotranslational targeting of proteins to the endoplasmic reticulum (ER). The SRP Alu domain is specifically required for delaying nascent chain elongation upon signal sequence recognition by SRP and was therefore proposed to interact directly with ribosomes. Using protein cross-linking, we provide experimental evidence that the A...
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The signal recognition particle (SRP) from Escherichia coli consists of 4.5S RNA and protein Ffh. It is essential for targeting ribosomes that are translating integral membrane proteins to the translocation pore in the plasma membrane. Independently of Ffh, 4.5S RNA also interacts with elongation factor G (EF-G) and the 30S ribosomal subunit. Here we use a cross-linking approach to probe the co...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2004
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi0353777